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KMID : 0644120110240030001
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Journal of Oriental Gynecology
2011 Volume.24 No. 3 p.1 ~ p.13
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Study of Apoptosis by Scirpi Tuber in Hela Cell and MCF-7 Cell
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Ryoo Gap-Soon
Lee Jin-Moo
Lee Chang-Hoon
Jang Jun-Bock
Lee Kyung-Sub
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Abstract
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Objectives:This study was designed to investigate the analysis of apoptosis by Scirpi Tuber in Hela cell and MCF-7 cell.
Methods:For cytotoxic effect of Scirpi Tuber extract, Scirpi Tuber extract were cultured on NIH3T3 cell in vitro. After treatment with various concentration of Scirpi Tuber, cell growth was evaluated in Hela cell and MCF-7 cell. Hoechst 33342 staining was performed to estimate DNA fragment effect of Scirpi Tuber on the apoptosis in Hela cell and MCF-7 cell. Annexin V/PI apoptosis assay was used to estimate the effects of Scirpi Tuber on the early apoptosis in Hela cell MCF-7 cell. All the stained cells were analyzed by a FACS. RT-PCR was used to estimate the apoptosis gene expression effect of Scirpi Tuber extract on Hela cell and MCF-7 cell.
Results:Cytotoxic effect of Scirpi Tuber extract was not found on per NIH3T3 cell. The viability of Hela cell was significantly decreased Scirpi Tuber (500, 1,000㎍/㎖) in Hela cell 1day, 3day and 5days after treatment (p<0.01). The viability of MCF-7 cell was significantly decresed Scirpi Tuber (1,000㎍/㎖) in MCF-7 cell (p<0.01), Scirpi Tuber (500㎍/㎖) in MCF-7 cell only 3days after treatment (p<0.01). In RT-PCR analysis, after treatment of 100㎍/㎖ of ACR extract, BCL-2 were decreased and BAX, caspase-3 were increased both in Hela cell and MCF-7 cell. DNA fragmentation was observed the Scirpi Tuber on Hela cell and MCF-7 cell. As time goes on DNA fragmentation incresed. In Annexin V/PI apoptosis assay, after treatment of 1㎎/㎖ of Scirpi Tuber, the early apoptotic cell increased both in Hela cell and MCF-7 cell. As time goes on apoptotic cell increased.
Conslusion:Scirpi Tuber appears to have considerable activity on the apoptosis in Hela cell and MCF-7 cell.
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KEYWORD
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Scirpi Tuber, apoptosis, Hela cell and MCF-7 cell, BCL-2, BAX, caspase-3
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